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Katsumasa FUJITA (Osaka University)

HeLa cells monitored in the living state with a high-speed Raman microscope

Title: Raman microscopy for imaging cellular dynamics
Author: Katsumasa FUJITA
Affiliation: Associate Professor, Osaka University
Email: fujita[at]ap.eng.osaka-u.ac.jp
URL: http://lasie.ap.eng.osaka-u.ac.jp/ap1g1kat/
Abstract:

Raman images of living HeLa cells

Spontaneous Raman scattering is a weak optical effect and had not been used for biological imaging due to the requirement of a long exposure for the measurement. We have built an optical system that measures Raman spectra simultaneously at multiple points in a sample and constructed a high-speed Raman microscope, enabling label-free observation of living cells by using molecular vibrations as image contrast. The above figure shows a Raman image of living HeLa cells reconstructed by plotting Raman signal assigned to cytochrome c and protein beta-sheet and lipids. By using the Raman microscope, we have demonstrated the observations of molecular dynamics during cell division, apoptosis, and differentiation.
Biographical Sketch: Katsumasa Fujita has received his PhD in Applied Physics, Graduate School of Engineering, Osaka University, Japan in 2000. He has been working on development of laser microscopes using nonlinear optical phenomena for imaging biological specimens. From 2007, he has been an associate professor in Department of Applied Physics, Osaka University. His recent research interests are super-resolution microscopy and Raman/SERS microscopy for visualization of biological cells/tissues and their functions.

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